5. Laboratoř pro vysokoúčinnou kapalinovou chromatografii (HPLC)


High-Performance Liquid Chromatography (HPLC) makes use of a high-pressure pump to deliver a mobile phase solvent at a uniform rate and wide range of pressure (500 to 5000 psi). The most obvious advantage of HPLC over gravity liquid chromatography is that samples can be separated much quicker. In addition, samples that are not volatile or that would thermally decompose in gas chromatography, can be readily and routinely separated in HPLC. HPLC is accomplished by injection of a small amount of liquid sample into a moving stream of liquid (termed the mobile phase) that passes through a column packed with particles of a stationary phase. As in gas chromatography, separation of a mixture into its components depends on different degrees of retention of each component in the column. The extent to which a component is retained in the column is determined by its partitioning between the liquid mobile phase and the stationary phases. A variety of HPLC separation techniques that utilize different stationery and mobile phases have been developed.

Assistance and Services

HPLC laboratory supports all research involving analytical and semipreparative HPLC, spectrophotometry and ELISA assays The laboratory is capable of analyzing a wide variety of samples (from low m.w. compounds to proteins) and chemical sensing using UV-VIS or fluorescence detection, spectral analyses or immunochemical techniques. The laboratory is also open for providing services for outside users on terms of scientific cooperation.

Equipment and Methodologies

• Hewlett Packard 1100 Binary HPLC pump, Dionex P580 Quarternary pump with DA detector and Gilson FC203B fraction collector

• Dionex ultimate 3000 HPLC systém with Quarternary pump, Autosampler, DA detector, FL detector

• Thermo Scientific Evolution 201 UV-VIS spectrophotometer with Peltier thermostat and 8-position Cell changer

• Tecan Infinite M200 Pro Multimode Microplate Reader (UV-VIS absorbance, luminescence, fluorescence, time-delayed fluorescence reading modes) with Reagent Injectors and temperature control systems

• Thermo Scientific WellWash 4MK2 automatic microplate washer

• Hettich 320R Table-top refrigerated centrifuge  

Reservation Contact

Ing. Jitka Jechová
E-mail: jitka.jechova@nudz.cz

Tel: +420 283 088 243

Research and Technical Contact

Jan Říčný, Ph.D.

E-mail: jan.ricny@nudz.cz

Tel: 420 283 088 217

 


 


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